Novel ex-vivo mechanobiological intervertebral disc culture system
نویسندگان
چکیده
منابع مشابه
Mechanobiological Models for Intervertebral Disc Tissue Engineering
Low back pain, which is often caused by disc degeneration, is a major health problem. Due to the limitations of the current treatments for degenerative disc disease, tissue engineering methods have been proposed. These methods present the opportunity to restore the functionality of the intervertebral disc by repairing or replacing the degenerated tissue. One of the major challenges in intervert...
متن کاملBiomechanical disc culture system: feasibility study using rat intervertebral discs.
A small-scale biomechanical disc culture system was designed to stimulate intervertebral disc (IVD) 'motion segment' in culture environment with load-controlled compression and combined load (compression+shear). After 7 days of diurnal mechanical loading, cell viability of discs stimulated with static compression load (0.25 MPa) and static combined load (compression (0.25 MPa)+shear (1.5N)) wer...
متن کاملDevelopment of a whole disc organ culture system to study human intervertebral disc
STUDY TYPE Basic science Objective: Low back pain is one of the most common health problems1 and is strongly associated with intervertebral disc degeneration, (IVD). Current treatments remove the symptoms without reversing or even retarding the underlying problem. Development of new therapy for the regeneration of the degenerative IVD is complicated by the lack of a validated long-term organ ...
متن کاملGenetic and Functional Studies of the Intervertebral Disc: A Novel Murine Intervertebral Disc Model
Intervertebral disc (IVD) homeostasis is mediated through a combination of micro-environmental and biomechanical factors, all of which are subject to genetic influences. The aim of this study is to develop and characterize a genetically tractable, ex vivo organ culture model that can be used to further elucidate mechanisms of intervertebral disc disease. Specifically, we demonstrate that IVD di...
متن کاملRapid ex vivo expansion of human umbilical cord hematopoietic progenitors using a novel culture system.
Cell numbers limit the widespread clinical use of cord blood (CB) for gene therapy and marrow replacement in adults; a simple and effective method for ex vivo expansion of CB primitive progenitor cells (PPC) is required. Recently, the combination of thrombopoietin (TPO) and Flk-2/Flt-3 ligand (FL-2) was reported to support slow proliferation of CB-PPC in stroma-free liquid culture. We establish...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biomechanics
سال: 2012
ISSN: 0021-9290
DOI: 10.1016/j.jbiomech.2011.10.036